Article Document Academic Article Information Content Entity Continuant Continuant Journal Article Entity Entity Generically Dependent Continuant 2025-05-06T12:53:35 RDF description of Eye-specific gene expression following embryonic ethanol exposure in zebrafish: roles for heat shock factor 1 - http://repository.healthpartners.com/individual/document-rn21171 Animal Studies public Eye 43 28476 Reproductive Toxicology document-rn21171 2022-02-21T22:48:57.408-06:00 Eye-specific gene expression following embryonic ethanol exposure in zebrafish: roles for heat shock factor 1 <p>The mechanisms through which ethanol exposure results in developmental defects remain unclear. We used the zebrafish model to elucidate eye-specific mechanisms that underlie ethanol-mediated microphthalmia (reduced eye size), through time-series microarray analysis of gene expression within eyes of embryos exposed to 1.5% ethanol. 62 genes were differentially expressed (DE) in ethanol-treated as compared to control eyes sampled during retinal neurogenesis (24-48 h post-fertilization). The EDGE (extraction of differential gene expression) algorithm identified >3000 genes DE over developmental time in ethanol-exposed eyes as compared to controls. The DE lists included several genes indicating a mis-regulated cellular stress response due to ethanol exposure. Combined treatment with sub-threshold levels of ethanol and a morpholino targeting heat shock factor 1 mRNA resulted in microphthalmia, suggesting convergent molecular pathways. Thermal preconditioning partially prevented ethanol-mediated microphthalmia while maintaining Hsf-1 expression. These data suggest roles for reduced Hsf-1 in mediating microphthalmic effects of embryonic ethanol exposure.<p> Drugs and Drug Therapy 16988 Genetics 10.1016/j.reprotox.2013.12.002