Article Document Academic Article Information Content Entity Continuant Continuant Journal Article Entity Entity Generically Dependent Continuant 2025-05-08T02:48:58 RDF description of Immunogold labeling of Alzheimer paired helical filaments with ganglioside MAB A2B5 - http://repository.healthpartners.com/individual/document-rn20387 2022-02-21T22:48:57.408-06:00 public 27886 Alzheimer's Disease Alzheimer Disease and Associated Disorders Immunogold labeling of Alzheimer paired helical filaments with ganglioside MAB A2B5 document-rn20387 16683 10.1097/00002093-199040100-00004 1 <p>The ganglioside monoclonal antibody A2B5 has previously been used at the light microscopic level to label Alzheimer neurofibrillary tangles (NFTs). Light microscopic analysis, however, could not reveal whether the A2B5 antibody-labeled NFTs or membrane fragments associated with NFTs. Therefore, we used pre-embedding immunohistochemical electron microscopy to examine A2B5 labeling of NFT. We found that the A2B5 antibody does indeed label a NFT antigen associated with the paired helical filament (PHF) structure, while no significant labeling of membranes or membrane fragments was observed. However, no clear periodicity of the immunogold label on the PHF was found.<p> Brain 4