Article Document Academic Article Information Content Entity Continuant Continuant Journal Article Entity Entity Generically Dependent Continuant 2025-05-08T04:49:02 RDF description of Inhibitor of antagonist binding to the muscarinic receptor is elevated in Alzheimer's brain - http://repository.healthpartners.com/individual/document-rn20369 Brain 16721 10.1016/0006-8993(94)91609-8 public 1-2 2022-02-21T22:48:57.408-06:00 Drugs and Drug Therapy 27962 655 Alzheimer's Disease <p>The 100,000 x g supernatant fraction of human brain contains endogenous inhibitors of antagonist binding to the muscarinic receptor. Significantly greater inhibition was observed with Alzheimer's than non-demented control supernatant fractions. Low molecular weight inhibitor was separated from larger inhibitor species by membrane dialysis (3,500 dalton cut-off). The activity of low molecular weight inhibitor was greatly increased by sulfhydryl reducing agents. While the low molecular weight inhibitor was stable to heat, acid and base for short time periods (< 20 min), it was inactivated by acid hydrolysis (50% loss after 16 h, 100% loss after 96 h). The low molecular weight inhibitor activity is elevated approximately three-fold in Alzheimer's brain. The low molecular weight inhibitor from Alzheimer's brain was found to be a non-competitive inhibitor. This is the first report of endogenous inhibitors in human brain of ligand binding to the muscarinic receptor and of increased inhibitor activity in Alzheimer's disease.<p> document-rn20369 Brain Research Inhibitor of antagonist binding to the muscarinic receptor is elevated in Alzheimer's brain